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Using the rat RBL-2H3 mast cell line as model, we have found that a panel of NO donors, of varying chemical type and half-life, each produced a time-dependent, and in some cases reversible, inhibition of IgE/antigen-induced expression of mRNA for TNF, IL-4 and IL-6 Davis et al

An Ultimate 3000 UHPLC (Dionex) was coupled to the Q Exactive-Mass plus spectrometer (QE-MS, Thermo Scientific) for metabolite separation and detection